茨城大学
理学部
理学科(生物科学コース)

顔写真
教授

仁木 雄三

ニキ ユウゾウ
NIKI Yuzo
  • 1953年生まれ

学歴

  1. 東京教育大学 理学部 生物学科動物学専攻 1975 卒業
  2. 筑波大学大学院博士課程 生物科学研究科生物学専攻 博士 1979 中退

学位

  1. 理学博士 筑波大学生物科学系

教育・研究活動状況

キイロショウジョウバエの始原生殖細胞および生殖幹細胞に関する研究。in vitroにおけるニッチ形成と成長因子の役割。

研究分野

  1. 発生生物学

研究キーワード

  1. ショウジョウバエを用いて、発生過程における始原生殖細胞・生殖幹細胞の形成に関与している遺伝子、シグナル伝達、ニッチ形成を研究している。特に、生殖細胞およびニッチ細胞の安定的な細胞株を樹立し、in vitro系での解析を行っている。

研究テーマ

  1. ショウジョウバエの始原生殖細胞および生殖幹細胞のin vitroでの解析 1998-2010
  2. 培養系を用いたショウジョウバエGSC/ニッチ・システムの解明と分化系の開発 2008-2013

著書

  1. Drosophila Germline Stem Cells for In Vitro Analyses of PIWI-mediated RNAi In Vitro Analyses of PIWI-mediated RNAi. in "PIWI-interacting RNAs" M. C. Siomi, (Ed): Methods and Protocols the Methods in Molecular Biology Vol.1093 Humana Press 2014
  2. Culturing Ovarian Somatic and Germline Stem Cells of Drosophila. In "Current Protocols in Stem Cell Biology 10, 2E.1.1-2E.1.9." John Wiley & Sons, Inc. 2009
  3. In vitro approaches of germline stem cells in fly and mouse In Stem Cell Applications in Disease and Health “ Eds. W.B. Burnsides et al.) New York Yuzo Niki Nova Science Publishers, Inc. 2008 978-1-60456-255-2
  4. Mitochondrial mediated transformation of Drosophila. In "Mitochondrial genetics and biogenetics", (ed. M. Attardi & A. Choomyn) Methods in Enzymology Vol.264 E. T. Matsuura and Y. Niki Academic Press (Elsevier) 1994
  5. ショウジョウバエ配偶子幹細胞/ニッチの試験管内での再構築と分化誘導 細胞工学 27巻 仁木雄三 学研メディカル秀潤社 2010

論文

  1. 研究論文(学術雑誌) 共著 Stable transformation and cloning mediated by piggyBac vector and RNA interference knockdown of Drosophila ovarian cell line. Hiroshi Uetake, Kenji Oka, and Yuzo Niki In Vitro Cellular & Developmental Biology – Animal 47, 689-694 2011 10.1007/s11626-011-9463-y An in vitro study is a powerful method for elucidating gene functions in cellular and developmental events. However, until date, no reliable in vitro transformation, cloning, or knockdown system has been reported for Drosophila cells, with the exception of S2 and Kc cells. In this study, we demonstrated that the piggyBac vector stably integrates donor DNA into ovarian somatic sheets derived from follicle stem cells. The transformed ovarian somatic sheet cells were easily cloned with a new piggyBac selection vector carrying enhanced green fluorescent protein and dihydrofolate reductase genes, egfp, and dhfr, respectively, in culture media containing methotrexate, an inhibitor of DNA synthesis. Donor egfp continued to be expressed at a high level in long-term culture. Furthermore, the translation of donor egfp was inhibited by treatment with double-stranded RNA derived from the target gene. The transfection and cloning methods mediated by the piggyBac vector would thus be useful for future analyses of gene functions in OSS cells and possibly be applicable to other Drosophila cell lines.
  2. 研究論文(学術雑誌) 共著 Abundant primary piRNAs, endo-siRNAs and microRNAs in a Drosophila ovary cell line Nelson C. Lau, Nicolas Robine, Raquel Martin, Wei-Jen Chung, Yuzo Niki, Eugene Berezikov, and Eric C. Lai Genome Research  19 2009/06
  3. 研究論文(学術雑誌) 共著 Autonomous differentiation of Drosophila spermatogonia in vitro. Tatsuya Kawamoto, Kieta Kawai, Tatsuji Kodama, Tomohiro Yokokura, YuzoNiki. Dev. Growth Differ. 50, 623-632 2008
  4. 研究論文(学術雑誌) 共著 Wingless signaling initiates mitosis of primordial germ cells during development in Drosophila Takuya Sato, Sachie Ueda and Yuzo NIki Mechanism of Development 125 2008
  5. (MISC)総説・解説(その他) 単著 生殖幹細胞の分離と安定的な細胞株の樹立― ショウジョウバエのin vitro系の確立を目指して 羊土社 2007/05

研究発表

  1. 口頭発表(一般) Cellular and molecular analyses of a stable Drosophila germline stem cell niche reconstructed in vitro The 58th/60th NIBB Conference -Germline -Specification, Sex, and Stem Cells- 2012/07
  2. ポスター発表 in vitro analyses of cellular interactions among germline stem cells, cap cells and escort cells in Drosophila. The 53rd Annual Drosophila Research Conference (Chicago USA) 2012/03
  3. ポスター発表 Microarray Gene Expression Profiling of Germline Stem Cell Niche and Ovarian Somatic Cells in Drosophila melanogaster The 34th Annual Meeting of the MBSJ 2011/12
  4. ポスター発表 Ecdysone signaling during spermatogenesis in Drosophila The 34th Annual Meeting of the MBSJ 2011/12
  5. ポスター発表 in vitro analyses of male germline stem cells and their niche cells in Drosophila The 34th Annual Meeting of the MBSJ 2011/12

担当授業科目

  1. 発生生物学
  2. 一般生物実験
  3. 細胞・発生生物学実験
  4. 基礎生物学II

所属学協会

  1. 日本分子生物学会
  2. 日本節足動物発生学会
  3. 日本発生生物学会