著書

公開件数: 3 件
No. タイトル 著者 担当範囲 出版社 出版日 ISBN URL 概要
1 貧栄養下で芳香族化合物を分解できる新規光合成微生物
西澤明人、千田美紀、千田俊哉、菓子野康浩、福田雅夫、木村成伸

ケミカルエンジニヤリング
2009/10/01



2 反応中間体構造から明らかになった電子伝達タンパク質間の酸化還元依存的な親和性調節機構
千田美紀, 木村成伸, 福田雅夫, 石田哲夫, 千田俊哉

日本結晶学会誌
2008/12


Redox-dependent affinity regulation is critical to fast and efficient electron transfer(ET)between ET proteins. The molecular mechanism of the affinity regulation, however, remains elusive due to the lack of crystal structures of the ET proteins in every redox state relevant to the ET reaction. BphA4 and BphA3 are, respectively, an FAD-containing NADH-dependent ferredoxin reductase and a Rieske-type[2Fe-2S]ferredoxin of a biphenyl dioxygenase BphA derived from Acidovorax sp. strain KKS102. Our biochemical study showed that the reduction of the FAD in BphA4 increases the affinity between BphA3 and BphA4 approximately 20-fold. In order to reveal the molecular mechanism of this redox-dependent affinity regulation, we determined the crystal structure of the following molecular species: BphA4 in oxidized, hydroquinone, semiquinone, and reoxidized forms; BphA3 in oxidized and reduced forms; and the ET complex of BphA3 and BphA4. A comparative analysis of these seven crystal structures obtained revealed that a series of conformational changes of BphA4 occurs upon reduction of FAD to form a high-affinity BphA3-binding site in BphA4.
3 電子伝達タンパク質間の酸化還元状態依存的親和性調節機構
千田美紀、木村成伸、福田雅夫、石田哲夫、千田俊哉

化学と生物
2008/10